Composite

Part:BBa_K3450012

Designed by: Priya Sharma Shashaank G.   Group: iGEM20_IISER_Bhopal   (2020-10-23)

Phosphate-Sensitive Kill Switch: Allows E. coli to express E2-IM2 toxin-antitoxin system sensor

This composite part allows E. coli to express the E2-IM2 toxin-antitoxin system sensitive to a phosphate-responsive repressor. This kill switch makes use of the E2-IM2 toxin-antitoxin system to make the chassis’ survival contingent on the activity of the phosphate-starvation-inducible promoter PPhoA. This dependence is established using the bacteriophage repressor cI.Under conditions of phosphate starvation, cI is expressed, shutting down the expression of the antitoxin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 464
    Illegal NheI site found at 487
    Illegal NheI site found at 1825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 217
    Illegal BglII site found at 1394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 538
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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